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Scientific Oral Presentations

Otology/Neurotology

Session: Scientific Oral Presentations 8 - Otology/Neurotology

Quantitative Assessment of Collagen Architecture to Determine the Tumor Stroma's Role During Vestibular Schwannoma Progression

Tuesday, October 1, 2024
11:12 AM – 11:18 AM EDT
Location: MBCC, 231
Disclosure(s):
Melanie L. Fisher, BS:

faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

Oliver F. Adunka, MD, MBA:

faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

Introduction: The progression of vestibular schwannoma (VS) is influenced by interactions between schwannoma cells and their surrounding extracellular matrix (ECM). However, the evolution of the architecture of collagen fibers, the most abundant ECM component, during VS growth is largely unknown.

Methods: Tumors from patients with sporadic, non-irradiated VS undergoing microsurgical resection were collected and stained with picrosirius red and examined under polarized light microscopy to identify collagen in tumors. An automated collagen fiber detection software (CT-FIRE) analyzed collagen architecture and topology. Specific collagen subtypes were validated in VS tissue by immunofluorescence.

Results: In total, 11 patients were included (average tumor diameter 2.8 cm, range 1.5-4 cm) and grouped into two cohorts, 5 with large VS and 6 with small tumors (3.5 cm vs 2 cm, p < 0.001). Tumor volume correlated with the density of thick collagen fibers representing mature Type I collagen (r2 = 0.395, p = 0.019) and thin fibers representing immature Type I or mature Type III collagen (r2 = 0.28, p = 0.047). Collagen fiber topology was significantly more disorganized in larger VS compared to small tumors, indicated by a higher proportion of misaligned collagen (76% vs 65% of fibers oriented >20 degrees away from median, p = 0.014). Collagen I intensity by immunofluorescence was significantly increased in large VS compared to small tumors (14-fold increase, p < 0.001) and to peripheral nerve control (>4-fold, p = 0.027).

Conclusions: Large VS is characterized by increased deposition of collagen with more disorganized architecture compared to small tumors and normal nerve. This highlights the potential role of collagen in the remodeling of ECM and proliferation of schwannomas.

    Presenting Author(s)

  • Melanie L. Fisher, BS

    Research Technician
    The Ohio State University Wexner Medical Center
    Columbus, Ohio, United States

    Disclosure(s):

    faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

    • Co-Author(s)

  • Bailey H. Duhon, MS

    Medical Student
    The Ohio State University Wexner Medical Center
    Columbus, OH, United States

    Disclosure information not submitted.

  • JT

    Jeffrey Tonniges, PhD

    The Ohio State University, United States

    Disclosure information not submitted.

  • DP

    Daniel M. Prevedello, MD, MBA

    The Ohio State University Wexner Medical Center, United States

    Disclosure information not submitted.

  • OA

    Oliver F. Adunka, MD, MBA

    Professor
    Ohio State University
    Columbus, OH, United States

    Disclosure(s):

    faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

  • KW

    Kyle Wu, MD

    The Ohio State University Wexner Medical Center, United States

    Disclosure information not submitted.

    • Senior Author(s)

  • YR

    Yin Ren, MD, PhD

    Assistant Professor
    Ohio State University
    Columbus, Ohio, United States

    Disclosure information not submitted.