Skip to main content
AAO-HNSF 2024 Annual Meeting & OTO EXPO Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.
Back

Scientific Oral Presentations

Head and Neck Surgery

Session: Scientific Oral Presentations 6 - Head and Neck Surgery

Altered Oral Bacteria Abundance Associated With Pathologic Features of Oral Squamous Cell Carcinoma

Monday, September 30, 2024
2:36 PM – 2:42 PM EDT
Location: MBCC, 208
Disclosure(s):
Delaney H. Sheehan, MD:

faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

Nicolaus D. Knight, MS:

faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

Panuganti A. Bharat, MD:

faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

Introduction: The oral microbiome is altered in the presence of oral squamous cell carcinoma (OSCC). This study aims to determine if there are microbiome differences based on pathologic features of OSCC tumors using The Cancer Microbiome Atlas (TCMA) and an in-tandem analysis of a prospectively collected database.

Methods: TCMA is a publicly available database containing curated, decontaminated microbial profiles for tumors from 1,772 patients; this study is limited to OSCC tumors. Separately, our institution collects oral swab samples from OSCC tumors prior to surgical treatment for 16S rRNA sequencing. Tumors with and without certain pathologic features were analyzed using logistic regression models reported with hazard ratio and 95% confidence interval.

Results: 105 patients with OSCC were included from TCMA with diverse oral cavity primary sites. 26% were Stage I-II and 74% were Stage III-IVA. Rates of lymphovascular invasion (LVI) were 23%, perineural invasion (PNI) 56% and microscopic or gross extranodal extension (ENE) 26%. There were 40 bacterial genera identified. Pseudopropionibacterium (p=0.0225) and Campylobacter (p=0.045) demonstrated significant changes in relative abundance in tumors with ENE versus no ENE as well as in tumors with LVI versus no LVI. There were no significantly enriched bacterial genera in tumors with PNI versus no PNI. Preliminary analysis of a prospectively collected database of OSCC microbiome oral swab samples showed significant differences in beta diversity for both ENE versus no ENE and LVI versus no LVI. The oral microbiome associated with ENE demonstrated changes in relative abundance of Campylobacter (p=0.0092) along with multiple other genera including Actinomyces (p=0.00011), Prevotella (p=0.017), Neisseria (p=0.013), Veillonella (p=0.0075), and Haemophilus (p=0.0044). Significantly altered bacteria associated with LVI included Prevotella (p=1.1e-05), Neisseria (p=3e-04), Streptococcus (p=0.0088), Fusobacterium (p=0.0026), Lactobacillus (p=0.00029), and Corynebacterium (p=0.00026).

Conclusions: Changes in the relative abundance of select oral bacteria genera are associated with adverse pathologic features of OSCC including ENE and LVI.

    Presenting Author(s)

  • Delaney H. Sheehan, MD

    Resident
    Department of Otolaryngology – Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, AL
    Birmingham, AL, United States

    Disclosure(s):

    faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

    • Co-Author(s)

  • NK

    Nicolaus D. Knight, MS

    Clinical Data Specialist
    Department of Otolaryngology - Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, AL
    Pinson, AL, United States

    Disclosure(s):

    faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

  • JP

    Juhi J. Patel, BS

    Researcher II
    4Department of Otolaryngology – Head and Neck Surgery, University of Alabama at Birmingham
    Madison, AL, United States

    Disclosure information not submitted.

  • PM

    Patrick Molina, PhD

    Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States

    Disclosure information not submitted.

  • NY

    Nengjun Yi, PhD

    School of Public Health, University of Alabama at Birmingham, Birmingham, AL, United States

    Disclosure information not submitted.

  • PB

    Panuganti A. Bharat, MD

    Assistant Professor
    University of Alabama at Birmingham
    Birmingham, AL, United States

    Disclosure(s):

    faculty for this accredited education activity has no relationship(s) with ineligible companies to disclose.

    • Senior Author(s)

  • Carissa M. Thomas, MD, PhD

    Assistant Professor
    University of Alabama at Birmingham
    Birmingham, AL, United States

    Disclosure information not submitted.